Ebola Virus Optical Pooled Screen | Genome-wide Screen Images

Overview This archive contains a ~10 % curated subset of genome‑wide phenotyping images. Images are from plate GWA (wells A1, A2, A3, B1, B2) in HeLa cells with a genome-wide CRISPR knockout library (~20,0000 genes, 4 sgRNAs/gene). Data are read out by optical pooled screening. Image Locations Genome‑wide phenotyping: gs://opspublic-east1/EBOVOpticalPooledScreen/Images/GW[A-I] (Each letter represents a distinct 6‑well plate; plate I has wells A1/A2 only.) Metadata: gs://opspublic-east1/EBOVOpticalPooledScreen/Metadata/Genomewide Secondary screen (for reference): gs://opspublic-east1/EBOVOpticalPooledScreen/Images/SEC_[Early,Late] Metadata: .../Metadata/Secondary Phenotyping Channels (Genome‑Wide) Channel 1: DAPI (nuclei) Channel 2: Ebola VP35 RNA FISH (positive‑sense) Channel 3: Ebola VP35 protein Channel 4: c‑Jun Channel 5: Vimentin Channel 6: LAMP1 Manuscript Context Filoviruses such as Ebola virus (EBOV) cause recurrent outbreaks with high mortality, yet therapeutic options remain scarce. Using an image‑based, genome‑wide CRISPR knockout screen spanning 39 million cells, we identified 998 host genes that modulate distinct stages of the EBOV life‑cycle. Key validated hits UQCRB – Post‑entry regulator of viral RNA synthesis; small‑molecule inhibition lowers EBOV titres in vitro. STRAP – Spliceosome‑associated factor whose disruption skews the viral RNA‑to‑protein ratio; interacts with viral co‑factor VP35. The primary screen plus 12 targeted secondary screens (including Sudan and Marburg virus assays) provide a rich resource for host dependencies and potential antiviral targets.