Transcriptome analysis of hiHepPCs in various culture conditions, human liver-derived hepatocytes, human liver-derived cholangiocytes, HUVECs, and HPBECs

Transcriptome analysis of human induced hepatic progenitor cells (hiHepPCs) in various culture conditions, human liver-derived hepatocytes, human liver-derived cholangiocytes, human umbilical vein endothelial cells (HUVECs), and human peripheral blood-derived endothelial cells (HPBECs) We found that a specific combination of three transcription factors, FOXA3, HNF1A, and HNF6, could convert HUVECs and HPBECs into cells that closely resembled hepatic progenitor cells in vitro. These hiHepPCs were expandable in long-term culture and able to differentiate into hepatocytes and cholangiocytes in accordance with their culture conditions. We conducted RNA-seq analyses to investigate the characteristincs of hiHepPCs and their progenies, in addition to those of parental HUVECs, HPBECs, and human liver-derived cells. Overall design: RNA-seq analyses for HUVECs (HUVEC-1 and HUVEC-2), HUVEC-derived hiHepPCs in monolayer (HUVEC-hiHepPC-2D-1, HUVEC-hiHepPC-2D-2, HUVEC-hiHepPC-2D-3, HUVEC-hiHepPC-2D-P16-1, HUVEC-hiHepPC-2D-P16-2, and HUVEC-hiHepPC-2D-P16-3), cell aggregate (HUVEC-hiHepPC-aggregate-1, HUVEC-hiHepPC-aggregate-2, and HUVEC-hiHepPC-aggregate-3), and spheroid cultures (HUVEC-hiHepPC-sheroid-1, HUVEC-hiHepPC-sheroid-2, and HUVEC-hiHepPC-sheroid-3), HPBECs (HPBEC-1 and HPBEC-2), HPBEC-derived hiHepPCs in monolayer (HPBEC-hiHepPC-2D-1 and HPBEC-hiHepPC-2D-2), cell aggregate (HPBEC-hiHepPC-aggregate-1 and HPBEC-hiHepPC-aggregate-2), and spheroid cultures (HPBEC-hiHepPC-spheroid-1 and HPBEC-hiHepPC-spheroid-2), human cholangiocyte-derived spheroids (human-cholangiocyte-spheroid-culture), and non-cultured human hepatocytes (human-hepatocyte-1 and human-hepatocyte-2).