Identification of Ligand-Receptor Pairs that Drive Human Astrocyte Development

We tested a group of five neuronally-secreted ligands and validated their synergistic contributions to astrocyte development within both human cortical organoids and primary fetal tissue. Overall design: We performed differential gene expression and fold change analysis using data obtained from targeted and bulk RNA-seq of purified fetal astrocyte cell cultures and 3 different organoid lines at three time points.