Suplememtary Materials

Lai et al., Correlation Analysis Between IFIT2 and HLA Class II in Oral Cancer.Supplementary Table 1. Associations of IFIT2 and clinical variables in tumors from patients with oral cancer.Figure S1: There was no significant difference in the proliferation rate after <i>in vivo</i> inoculation. (A) Schematic illustrating the generation of the clonal sublines from CLA27cells. (B) Representative images of sublines after <i>in vivo</i> selection under phase contrast and fluorescence microscopy. Bars = 50 μm. (C) The proliferation rate was evaluated using the colony formation assay. Cells in logarithmic growth were harvested and reseeded (1,000 cells per 100-mm dish) in triplicate and incubated for 10 days. Colonies of more than 50 cells were counted after fixation in methanol and stained with 10% Giemsa solution.Figure S2: Flow chart of the microarray data analysis. ShCTRL: parental CAL27 cells expressing the lentiviral pGIPZ vector; shIFIT2-1, and -2: sublines of parental CAL27 cells expressing the lentiviral IFIT2-pGIPZ vector; shCTRL-T1 and T2: primary tumor clones derived from two shCTRL xenograft tissues; shIFIT2-T: primary tumor clone derived from shIFIT2 xenograft tissue; shIFIT2-M1 and -M2: different metastatic clones derived from two metastatic tumors derived from shIFIT2 cells after tail vein inoculation. Figure S3: mRNA levels of HLA class II molecules in HNC based on the sample type obtained from the TCGA database.Figure S3. mRNA levels of HLA class II molecules in HNC based on the sample type obtained from the TCGA database.