RNA sequencing of miRNA in plasma from myocardial ischemia and reperfusion mice and sham mice. RNA sequencing of miRNA in plasma from myocardial ischemia and reperfusion mice and sham mice

Purpose: The aim of this study is to compare the plasma miRNA profile between mice subject to myocardial ischemia and reperfusion and mice subject to sham operation. Methods: 8 to 10-week old C57BL/6 mice underwent myocardial ischemia and reperfusion (MIR) or Sham operation. Plasma RNA was isolated using Trizol LS reagent 4h post-surgery. NGS cDNA libraries were prepared using Norgen Biotek Small RNA Library Prep Kit. Library quality was validated prior to sequencing on an Illumina NextSeq 500 platform. Overall design: 8 to 10 weeks old male C57BL/6 mice were subject to myocardial ischemia and reperfusion (8 mice) or sham surgery (6 mice). Plasma was collect for RNA isolation 4 hour post-surgery. cDNA librarues were sequenced using Illumina NextSeq 500 platform.