Distinction of junctional cancers of gastric and oesophageal origin (DJANGO), batch#2

Aim of the study was the distinction of adeoncarcinomas at the gastroesophageal junction by their expression profile. Differential gene expression analysis between tumours of different Siewert type did not reveal relevant results. Stratification into three intrinsic subgroups was done by an diagonal distribution model using the mclust package in R. Clinicopathological and demographic parameters showed a similar distribution within the subgroups. Genes set ernichment analysis revealed different biological features for each subgroup that were also reflected by significant differences in the prognostic outcome for the patients, mainly overall survival and recurrence free survival. n=60, 49 gastro-oesophageal adenocarcinoma samples, 11 non-cancer control samples. (11 of the 49 cancer samples were technical replicates of samples also used in batch#1). adenocarcinoma samples: 35 GOJ (12 type 1, 13 type 2, 10 type 3), 6 distal body, 8 antrum. non-cancer control samples: 4 duodenum, 3 gastric body, 4 gastric cardia (matched samples from 4 individual patients).