RNA Sequencing Analysis of the epididymal adipose tissue from Normal Chow, HFD Control and SZ-A-treated HFD mice

Ramulus Mori (Sangzhi) alkaloids (SZ-A) improves lipid metabolism and adipose tissue inflammation in HFD-induced obese mice.This study compares transcriptome profiling (RNA-seq) in the epididymal adipose tissue of normal chow, high-fat diet (HFD) control and SZ-A-treated HFD mice to verify the regulatory mechanisms of SZ-A. These results demonstrated that SZ-A regulates lipid metabolism and inflammation. 6-week-old C57BL/6J mice were fed with normal chow or HFD for 14 weeks, and the HFD mice were treated with saline or SZ-A for another 6 weeks. We performed RNA-Sequencing of the epididymal adipose tissue of normal chow, HFD control and SZ-A-treated HFD mice (control group n=6, HFD and SZ-A groups n=7).