Data_Sheet_1_Supramolecular Fluorescence Probe Based on Twisted Cucurbit[14]uril for Sensing Fungicide Flusilazole.doc

The host-guest complex of the common dye, thioflavin T (ThT), and twisted cucurbit[14]uril (tQ[14]) was selected as a fluorescent probe to determine non-fluorescent triazole fungicides, including flusilazole, azaconazole, triadimefon, tebuconazole, tricyclazole, flutriafol, penconazole, and triadimenol isomer A, in an aqueous solution. The experimental results reveal that the ThT@tQ[14] probe selectively responded to flusilazole with significant fluorescence quenching and a detection limit of 1.27 × 10−8 mol/L. In addition, the response mechanism involves not only a cooperation interaction—ThT occupies a side-cavity of the tQ[14] host and the triazole fungicide occupies another side-cavity of the tQ[14] host—but also a competition interaction in which both ThT and the triazole fungicide occupy the side-cavities of the tQ[14] host.

本实验选取了常见染料硫堇T（ThT）与扭曲的环状[14]脲（tQ[14]）形成的宿主-客体复合物作为荧光探针，以检测水溶液中非荧光的三唑类杀菌剂，包括氟硅唑、咪鲜胺、三唑酮、丙环唑、三环唑、氟菌唑、苯醚甲环唑和三唑咪醇异构体A。实验结果显示，ThT@tQ[14]探针对氟硅唑表现出显著的荧光猝灭，检测限为1.27 × 10−8 mol/L。此外，响应机制不仅涉及ThT占据tQ[14]宿主分子的一侧空腔，而三唑类杀菌剂占据另一侧空腔的协同作用，还包括ThT与三唑类杀菌剂在占据tQ[14]宿主分子一侧空腔时的竞争作用。