Additional file 1: Table S1. of Fluorescence activated cell sorting followed by small RNA sequencing reveals stable microRNA expression during cell cycle progression

Characterization of cell cycle sorted cells and isolated RNA quantity in various cell types. Purity of cell cycle sort was determined by re-analyzing the sorted populations by FACS analysis: percentage was determined by the portion of cells residing in the gate previously designated for a certain cell cycle phase. Data of four replicate experiments. Data are given as × 1000 cells (sorted cells) and are shown as mean ± standard deviation. Table S2. Name and details of primers used for mRNA and miRNA expression qRT-PCR measurements. mRNA primers (Panel A, cat. No: 4331182) and miRNA primers (Panel B, cat. No: 4427975). All primers were from Applied Biosystems by Life Technologies. Table S3. Normalized expression of differently expressed genes between cell cycle phases in various cell types. Normalized expression of genes with fold change > 2 between cell cycle phases detected in HDFa cells (Panel A). Normalized expression of significantly differently expressed genes in cell cycle phases detected in NCI-H295R (Panel B) and HeLa (Panel C) cells. Note: For Panel B and C, genes are listed in the manner as shown in the heat map (Fig. 2, panel A and B, respectively). Table S4. List of genes shown on Venn diagram (Fig. 3, panel a). Genes are marked with “1” if being found cycling by either method (HDFa SORT, PF synchr, HeLa SORT, HeLa synchr). Gene IDs are Gene Symbols, if available or probe IDs. Table S5. List of HeLa cell cycle genes being present in enriched GO terms. Table 1 presents GO terms which are enriched in gene lists unique to HeLa SORT, HeLa synchronization experiments and the overlap beween HeLa SORT and synchronization lists. Gene symbols of genes being present in the gene lists and the enriched GO terms are shown here (HeLa SORT \ HeLa synchr - Panel A; HeLa synchr \ HeLa SORT – Panel B; HeLa SORT ∩ HeLa synchr – Panel C). (XLS 743 kb)