Genomic and single-cell landscape of transformed cutaneous T-cell lymphoma

Cutaneous T-cell lymphoma (CTCL) is a malignancy of skin-homing T cells. A subgroup of patients develops large cell transformation with progression to an aggressive lymphoma and with poor survival. We aimed to study the transformed CTCL (tCTCL) ecosystem using integrative approaches spanning whole-exome sequencing (WES), single-cell RNAseq, and immune profiling in a unique cohort of 56 patients with tCTCL We collected 16 fresh skin biopsies from 8 patients with transformed CTCL, each with paired PP and TT, and profiled these cells using complementary 5′ scRNA-seq and scV(D)J-seq strategies