RNA-Seq of human cell lines, MCF-7, HepG2 and HepaRG treated with a dose range of Coumarin from 0.001uM to 200uM against untreated vehicle controls

The application of RNA-seq profiling of cell lines following compound treatment has been highlighted as a line of evidence for Next Generation Risk Assessment of ingredients. We demonstrate as part of a use case the evaluation of a targeted RNAseq using Tempo-Seq platform as a general unbiased approach to complement more targeted approaches. We show impact of the use of different cell lines to the point of departure.