File S1 - CAV1 Promotes HCC Cell Progression and Metastasis through Wnt/β-Catenin Pathway

Supporting information. Figure S1. Subcellular localization of CAV1 was examined by immunofluorescence microscopy in HepG2 cells. Figure S2. CAV1 knockdown inhibit HCC tumor growth and metastasis in MHCC97H cells. A, B, shRNA-CAV1-1was used to knockdown CAV1 in MHCC97H cells, as demonstrated by real time RT-PCR and western blotting, where noshRNA was used as control. C, D, Cells (5×106) in 0.2 mL PBS were injected s.c. into the right upper flank region of each of 6 nude mice. The growth of subcutaneous tumor was recorded for 30 days, tumor growth was monitored for 3 days by measuring the tumor diameters (mean ± SD) then the mice were killed and tumors were removed. E, orthotopic implantation was assayed using small piece of subcutaneous tumor. The mice were observed for 6 weeks, killed, and autopsied. Tumors were weighed at 6 weeks; the weight is indicated (mean ± SD). F, G, Representative images of metastases that formed in lungs of each nude mouse (n = 6) at 6 weeks after orthotopicly implanted with MHCC97H no-shRNA or shRNA-CAV1-1 tumors. Black arrows indicate metastatic tumors in lung. Original magnification, ×100. (DOC)