KRAS Pyrosequencing analysis of cases with discordant results between ASLNAqPCR and Sanger sequencing.

SSEQ, Sanger sequencing; ASLNA, allele specific quantitative PCR using 3′-locked nucleic acid modified primers (ASLNAqPCR); WT, wild type; NP, not performed due to lack of additional DNA; CRC, colonic adenocarcinoma; NSCLC, lung adenocarcinoma; met, metastatic; LN, lymph node; FNA, fine needle aspirate; PC, pancreatic carcinoma. aASLNAqPCR primers designed to identify only the seven most common codon 12–13 KRAS mutations, codon 61 KRAS and G12F mutations are not detectable. bReal time ASLNAqPCR quantitative data. cStatus post neoadjuvant chemo– and radiation therapy. dPercentage of the tumor/non neoplastic cells ratio estimated in the area dissected for DNA extraction.