Effect of PME-1 knockout on MEF gene expression

We investigated the role of PME-1 (protein phosphatase methylesterase-1) as a specific methylesterase for the critical cancer suppressor, protein phosphatase 2A (PP2A), and its potential contributions to the development and malignancy of various cancers. Notably, no gene expression database related to PME-1 deficiency had been previously reported. To address this knowledge gap, we conducted an analysis of the impact of PME-1 on gene expression using mouse embryonic fibroblasts (MEFs) isolated from both PME-1 wild-type (WT) and knockout (KO) mice. Overall design: PME-1 KO cells and their controls (WT) were collected 24 hours after seeding in DMEM (high glucose) medium and subjected to RNA-seq analysis.