Homo sapiens Raw sequence reads

Luteolin has demonstrated therapeutic potential against pulmonary fibrosis, yet the underlying miRNA-mediated regulatory mechanisms remain poorly defined. In this study, we employed next-generation sequencing to explore the effects of luteolin on TGF-beta1-induced fibrogenesis in MRC5 cells, with a focus on miRNA-mRNA interactions. Real-time PCR analysis confirmed that luteolin pretreatment significantly suppressed TGF-beta1-induced expression of key fibrotic markers, including TGFB1, ACTA2, COL1A1, and FN1, indicating a potent anti-fibrotic effect. Transcriptomic profiling revealed that luteolin reversed the expression of 692 genes dysregulated by TGF-beta1, with functional enrichment analyses highlighting significant pathways related to cellular movement and localization. Furthermore, integrated miRNA-mRNA network analysis identified eight negatively correlated pairs potentially involved in these processes. These findings provide new insights into the anti-fibrotic mechanisms of luteolin and underscore the role of miRNA-mRNA regulatory networks in pulmonary fibrosis. This study enhances our molecular understanding of fibrogenesis and supports the potential of luteolin as a modulator of post-transcriptional gene regulation in fibrosis.