HDAC3/4 mediates leptin-induced BMP-2 mRNA expressions in human primary chondrocytes.

(A) Cells were pretreated with DMSO or TSA and then kept as controls or treated with leptin for 4 and 8 h. The BMP-2 mRNA expression was determined by real-time PCR. (B–C). Cells were kept as controls or treated with leptin for 1, 4, 8 and 24 h. (B) The HDACs expressions were determined by Western blot. (C) The subcellular localization of HDAC3 and 4 were determined by cell fractionation assay and Western blot. (D) Cells were transfected with HDAC3- or HDAC4-specific siRNA and then kept as controls or treated with leptin for 4 h. The BMP-2 mRNA expression was determined by real-time PCR. Data in (A) and (D) are mean ± SEM from three independent experiments. *, P vs. DMSO/control, siCL/control or untreated cells. #, P vs. DMSO/ or siCL/leptin-treated cells. Results in (B) and (C) are representative of three independent experiments with similar results.