The role of NOXO1 in the mouse model of cigarette smoke-induced emphysema and pulmonary hypertension

NOXO1 KO mice seem to be protected from cigarette smoke-induced emphysema and pulmonary hypertension. Since the molecular mechanisms are not clear yet, this microarray experiment should help to identify the molecular differences in these KO mice compared to the wild-type (WT), figuring out how the protection occurs. Since we believe that vascular altertions could not only be responsible for vascular remodeling but also the trigger for emphysema development, we microdissected pre-capillary vessels from WT and KO mice, both controls and smoke-exposed. In addition, alveolar septa were microdissected to investigate the alveolar changes. Single-color hybridization of RNA from microdissected lung tissues from 40 mice in total. From each mouse, septa and vessels were used. Four arrays were not analyzed because there was no signal.