Chaotic fluctuations in mitochondrial function under oxidative stress. SOD2 concentrations of 0.016 mM

These chaotic time series of mitochondrial dynamics under conditions of oxidative stress are analyzed in detail in Kembro et al. 2018. Mitochondrial chaotic dynamics: Redox-energetic behavior at the edge of stability. Sci. Rep. (in press).<br>Mitochondrial model used was described in the project overview and in detail (Kembro et al. 2013. Biophys J 104(2):332-343; Kembro et al., 2014. Front Physiol 5:257).<br><b>Analytical methods. </b>Numerical integration of the ME-R model equations was performed with MatCont 2.4 in MATLAB 7.1, until steady-state solutions were obtained (i.e., when the magnitude of each time derivative was -10). Time series with duration of at least 6e⁶ ms were constructed by numerical integration of model equations. To allow transient states to vanish, the system was computed during a time lapse of 2 e⁸ ms.All studies were performed using the parameter setting optimized in our previous work (Kembro et al. 2013. Biophys J 104(2):332-343; Kembro et al., 2014. Front Physiol 5:257), with ADPm = 0.01mM, i.e. consistent with energized mitochondria under state 4 respiration.The two .txt file represents the time reference and the time series of variables output of the model, in order from left to right column: 1) Mitochondrial Ca+;2) Mitochondrial ADP; 3) Membrane potential; 4)Mitocondrial NADH; 5) Mitochondrial H+; 6) Mitochondrial Phosfate, Pi; 7) Isocitrate; 8) a-ketoglutarate; 9) Succinyl CoA; 10) Succinate; 11) Fumarate; 12) Malate; 13) Oxaloacetate; 14) NADPH; 15) Mitochondrial superoxide; 16) Extramitochondrial superoxide; 17) Mitochondrial hydrogen peroxide; 18) Extramitochondrial hydrogen peroxide; 19) Mitochondrial GSH; 20) Extramitochondrial GSH; 21)Mitochondrial GSSG; 22) Mitochondrial TrxSH2; 23) ExtramitochondrialTrxSH; 24)Mitochondrial PSSGm; 25) Extramitochondrial PSSG.<br>The parameter settings were:Model-simulated time series were calculated with 0.016 mM of SOD2, Shunt=0.04, SOD1 9.7 10^-5 mM. External superoxide perturbation: amplitude=1 10^-7 mM, period =30 s.