CSPP1-E1A attenuates the ability of cap3A mutant S. pneumoniae to acquire capsule gene during natural transformation in mouse peritoneal cavity.
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Mice were intraperitoneally infected with S. pneumoniae cap3A mutant bacteria (3×109, 100 µl volume) supplemented with 100 µg of chromosomal DNA from the parental wild-type strain 0100993 in the presence or absence of 100 µg CSP1-E1A or CSP1-R3A. Mouse spleens were harvested at 24 hr post-infection, homogenized, serially diluted and plated on THB agar for enumeration of mucoid colonies.
创建时间：
2015-12-02
