bulk mRNA sequencing of splenic Red Pulp Fibroblasts (RPF) and lymph node Fibroblastic Reticular Cells (FRC)

bulk mRNA sequencing was used to compare the fibroblastic nature of RPF and FRC transcriptomes, and the transcriptionnal response induced in RPF by Red Pulp Macrophages depletion In total, 45,000 FRC were isolated from 78 lymph nodes from 6 untreated Wt1CreERT2 RosatdTomato CD64DTR mice, 45,000 RPF were isolated from 6 spleens from 6 untreated Wt1CreERT2 RosatdTomato CD64DTR mice and 45,000 RPF were isolated from 6 spleens from 6 diphteria-toxin-treated Wt1CreERT2 RosatdTomato CD64DTR mice. Each sample represents data from two mice.