PBMC were isolated 10 weeks post challenge from each macaque and cultured at 2×105 cells per well in triplicate wells of a 96 wells plate.
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PBMC were stimulated in vitro with 1 µg/ml of rBmHAT for 72 hrs at 37°C and analyzed for proliferation using cell counting kit (CCK-8). Con A was used as positive control. Stimulation index (S.I.) was calculated from the unstimulated control wells.Significant proliferation of PBMC **(PPBMC were isolated 10 weeks post challenge from each macaque and cultured at 2×105 cells per well in triplicate wells of a 96 wells plate.
创建时间：
2015-12-02
