Targeted Methyl-Capture Sequencing data from cord blood in infants with Autism Spectrum Disorder (ASD) vs. controls

DATASET DOCUMENTATION: DataMethylKit_Autism.xlsx Date: June 18, 2026 Institution: University of La Laguna (ULL) Author: Nieves Luisa González-González, Enrique González-Dávila Related Publication: Targeted Methyl-Capture Sequencing for the Discovery of Cord Blood DNA Methylation Biomarkers in Autism Spectrum Disorder: A Pilot Study This document describes the contents of the dataset file DataMethylKit_Autism.xlsx. This file contains the results of a DNA methylation analysis performed using the methylKit R package. All genomic coordinates are based on the hg19 reference genome. The data is organized into six worksheets detailing differentially methylated CpG sites and genomic regions. The first three worksheets contain the information for the comparison between infants later diagnosed with ASD vs. infants born to mothers with obesity and diabetes (IODM), and the last three worksheets are for comparing ASD vs. infants born to mothers with obesity and diabetes (IHM). Sheet 1 and 4: Bases_IODM and Bases_IHM This sheet lists individual CpG sites (single-cytosine resolution) that show statistically significant differences in methylation. · Chr (Chromosome): Genomic chromosome identifier. · Start / End: Genomic coordinates of the base. In this sheet, start and end values are identical as they represent a single nucleotide position. · p-value: Raw significance value obtained from the statistical test. · q-value: Adjusted p-value (False Discovery Rate, FDR) for multiple testing correction. · meth.diff: The difference in methylation levels expressed as a percentage (e.g., treatment group minus control group). Sheet 2 and 5: AnotBases_IOHM and AnotBases_IHM This sheet provides functional annotation for the CpG sites listed in the Bases tab. · Gene Name: Closest gene identifier using official RefSeq nomenclature (NM_... for mRNA or NR_... for non-coding RNA). Genome assembly hg19. · dist.to.feature: Physical distance (in base pairs) from the CpG position to the nearest genomic feature (e.g., Transcription Start Site). Sheet 3 and 6: Region_IOHM and Region_IHM This sheet contains data for genomic regions or segments (clusters of CpGs) showing significant methylation differences. · Chr (Chromosome): Genomic chromosome identifier. · Start: Starting genomic coordinate of the segment. · End: Ending genomic coordinate of the segment. Unlike the Bases tab, these values differ, defining the length of the region. · p-value / q-value / meth.diff: Statistical metrics equivalent to those described in the Bases tab, but calculated for the entire region/segment.