Transcriptome analysis of human embryonic stem cells (hESCs) and human fibroblasts upon rapamycin. Transcriptome analysis of human embryonic stem cells (hESCs) and human fibroblasts upon rapamycin

Rapamycin inhibits mTORC1 complex in a variety of cells. We demonstrated that hESCs are more sensitive to rapamycin treatment as compared to somatic cells (BJ Fibroblasts) and also certain cancer cells (KBM7 cells). To study the molecular mechanisms of this disparity, we performed a transcriptome analysis (RNAseq) of rapamycin-treated hESCs and human fibroblasts for two time points (2 and 4 days of treatment). Overall design: Human embryonic stem cells (hESCs) and human fibroblasts were treated with rapamycin for two and four days. For untreated controls and each treatment condition three biological replicates were analyzed.