An atlas of transcription factors expressed in the Drosophila melanogaster pupal terminalia

The male terminalia of Drosophila melanogaster has undergone rapid morphological change between it and its closely related species, making it a useful model for studying evolution and development. In order to identify the network components that pattern the male terminalia, we isolated and sequenced whole RNA from two timepoints during pupal development, and next performed in situ hybridization for 100 highly expressed transcription factors during pupal development. Overall design: RNA from 4 samples each from 24 hour APF and 28 hour APF D. melanogaster male pupal terminalia were isolated and sequenced using Illumina sequencing.