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Figure S7

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NIAID Data Ecosystem2026-05-10 收录
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https://figshare.com/articles/dataset/Figure_S7/30161791
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资源简介:
Western Blot raw images of p21 protein levels for untreated, irradiated, and neocarzinostatin treated M. m. musculus (PWK) and M. spretus (STF) primary fibroblasts. Primary antibodies included rabbit monoclonal Anti-p21 (ab188224, Abcam, 1:1000) and mouse monoclonal anti-β-tubulin (T9026, Sigma-Aldrich, 1:1000). Secondary antibodies used were Goat Anti-Mouse IgG(H+L) Human ads-HRP (Cat#1031-05, Southern Biotech, 1:5000) and Goat Anti-Rabbit IgG(H+L) Human ads-HRP (Cat#4050-05, Southern Biotech, 1:5000). Blots were developed using either Clarity Western ECL Substrate (Bio-Rad) or Radiance Plus Femtogram-sensitivity HRP Substrate (Azure Biosystems) and imaged on a Chemidoc MP imager (Bio-Rad).
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