CRISPRi/a screen of doxorubicin induced cardiotoxicity. CRISPRi/a screen of doxorubicin induced cardiotoxicity

Druggable genome of CRISPRi/a sgRNA libraries for druggable genes protecting doxorubicin induced cardiomyocyte toxicity using human induced pluripotent stem cell (iPSC) derived cardiomyocytes. Overall design: Vehicle (DMSO) and doxorubicin treated iPSC differentiated cardiomyocytes (iCMs). iCMs were transfected with sgRNA libraries and CRISPRi (dCas9-KRAB) or CRISPRa (dCas9-VPR). Two biological replicates were used in both CRISPRi and CRISPRa screens.