Data underlying the research of the Role of bacterial proteins in the onset of ANCA vasculitis

Goal: The aim of the project was to investigate whether S.aureus proteins SPIN and 6PGD via the formation of the hybrid auto-antigen SPIN:MPO or MPO mimitope 6PGD could explain the differential aetiologies of the disease GPA (Granulomatosis with polyangitis) caused by MPO-ANCA (Anti Neutrophil Cytoplasmic Autoantibodies).Methods: Isolation kinetic verification of S.Aureus 6PGD and SPIN by means of Activity measurements, see Readme for further specifications. Determination of antibody reactivity/titers using ELISA in sera from patients with MPO ANCA and healthy individuals against the hybrid auto-antigen MPO:ANCA or 6PGD, see Readme for further specifications. Data: Antibody titers ELISA and (Kinetics) colorigenic activity assays.<br>

研究目标：本项目旨在探究，金黄色葡萄球菌（Staphylococcus aureus）蛋白SPIN与6PGD通过形成杂交自身抗原SPIN:MPO，或是MPO模拟表位6PGD，能否解释由MPO-ANCA（抗中性粒细胞胞浆自身抗体，Anti Neutrophil Cytoplasmic Autoantibodies）引发的GPA（肉芽肿性多血管炎，Granulomatosis with polyangitis）的差异化病因学机制。
研究方法：通过活性测定法完成金黄色葡萄球菌6PGD与SPIN的分离动力学验证，详细参数详见自述文件（Readme）；采用酶联免疫吸附试验（ELISA，Enzyme-Linked Immunosorbent Assay）检测MPO-ANCA阳性患者与健康个体血清中，针对杂交自身抗原MPO:ANCA或6PGD的抗体反应性与抗体滴度，详细参数详见自述文件（Readme）。
数据集内容：包含抗体滴度ELISA检测结果及（动力学）显色活性测定结果。