Deep RNA-seq of Universal Human Reference RNA mixed with external spike in molecules ERCC mix 1 using prime-seq. This sample is anologous to sample A in the MAQC-III study, while the mixing ratios and the input was the same 1000th of the total input was used here.. Deep RNA-seq of Universal Human Reference RNA mixed with external spike in molecules ERCC mix 1 using prime-seq. This sample is anologous to sample A in the MAQC-III study, while the mixing ratios and the input was the same 1000th of the total input was used here.

Here a part of the MAQC-III study was repeated with the prime-seq method to have a dataset to compare to this gold standard RNA-seq dataset using power simulations.