Metabolomics data from: Dietary caloric input and tumor growth accelerate senescence and modulate liver and adipose tissue crosstalk

Metabolic alterations are related to tumorigenesis and other age-related diseases that are accelerated by “Westernized” diets. In fact, hypercaloric nutrition is associated with the increased incidence of cancers and faster aging. Conversely, lifespan-extending strategies, such as caloric restriction, impose beneficial effects on both processes. Here, we investigated the metabolic consequences of hypercaloric-induced aging on tumor growth in female mice. Our findings indicate that a high-fat high-sucrose diet increases tumor growth mainly due to the boosted oxidation of glucose and fatty acids. Consequently, through an increased expression of lactate, IGFBP3, and PTHLH, tumors modulate liver and white adipose tissue metabolism. In the liver, the induced tumor increases fibrosis and accelerates the senescence process, despite the lower systemic pro-inflammatory state. Importantly, the induced tumor induces the wasting and browning of white adipose tissue, thereby reversing diet-induced i..., Sample preparation
In a 2 mL centrifuge tube (Eppendorf), 50 µL of serum and 100 µL of ice-cold methanol with internal standard (p-fluoro-DL-phenylalanine and furosemide, final concentration of 2.5 µg/mL) were added. The mixture was homogenized by vortexing for 10 s, followed by an ultrasound bath with ice for 10 min and homogenized again for 10 s. Subsequently, the samples were incubated at -20 °C for 15 min for protein precipitation, followed by centrifugation at 10,000 xg for 15 min. In a new tube, the supernatant (120 µL) was collected and dried in a Turbovap equipment in a water bath at 45 °C and N2 gas pressure regulated at 7 psi for approximately 30 min. The dried extract was reconstituted in 60 µL of injection solvent (methanol:water, 1:1) with internal standard (Caffeine, final concentration of 1 µg/mL) and vortexed for 10 s. For solubilization, the reconstituted samples were placed in an ultrasonic bath for 5 min and centrifuged again at 10,000 x g for 5 min. Finally, an aliqu..., , # Metabolomics data from: Dietary caloric input and tumor growth accelerate senescence and modulate liver and adipose tissue crosstalk

[https://doi.org/10.5061/dryad.d7wm37qb3](https://doi.org/10.5061/dryad.d7wm37qb3)

#### Description of the data and file structure

In the attempt to evaluate whether the different treatments were altering circulating metabolites. For this, we prepared a pool of the sera from the different groups and proceeded with a metabolomic analysis of these samples. Sample preparation:  In a 2 mL centrifuge tube (Eppendorf), 50 µL of serum and 100 µL of ice-cold methanol with internal standard (p-fluoro-DL-phenylalanine and furosemide, final concentration of 2.5 µg/mL) were added. The mixture was homogenized by vortexing for 10 s, followed by an ultrasound bath with ice for 10 min and homogenized again for 10 s. Subsequently, the samples were incubated at -20 °C for 15 min for protein precipitation, followed by centrifugation at 10,000 xg for 15 min. In a new tub...