SoxB1 transcription factors are essential for intiating and maintaining neural plate border gene expression [RNA-seq]

Here we investigate a functional role for SoxB1 transcription factors, Sox2 and Sox3, on the transient embryonic cell population, the neural plate border. We find through gain/loss-of-function studies necessary for neural plate border cells to become neural crest cells. and genomics experiments (ChIP-seq and RNA-seq) that SoxB1 transcription factors directly promote neural plate border formation. and that down-regulation of SoxB1 expression is Overall design: We identify regions of Sox3 enrichment in blastula stem cells and neural plate border cells (St 11.5) through ChIP-seq studies. Furthermore, we assess changes in gene expression in soxb1 morphant (vs WT) blastula stem cells through RNA-seq.