Deep Phenotyping and Lifetime Trajectories Reveal Limited Effects of Longevity Regulators on the Aging Process

We employed large-scale phenotyping to analyze not only a few, but hundreds of phenotypes and thousands of molecular markers across tissues and organ systems in a single study in aging C57BL/6J mice. For each phenotype, we established lifetime profiles to determine at which age age-dependent phenotypic change is first detectable relative to the young adult baseline. The study includes young treated groups of animals, subjected to putative anti-aging interventions prior to the onset of age-dependent phenotypic change. In parallel, we assessed genetic variants for their effects on age-sensitive phenotypes in humans.本研究采用大规模表型分析（large-scale phenotyping）技术，针对自然衰老的C57BL/6J小鼠开展单一项研究，同步检测覆盖各组织与器官系统的数百种表型及数千个分子标记。针对每一种表型，我们构建了全生命周期特征谱，以确定相较于年轻成年基准组，年龄依赖性表型变化首次可被检出的具体年龄节点。本研究设置年轻受试动物干预组，在年龄依赖性表型变化出现前，对其施加潜在抗衰老干预手段。与此同时，我们还评估了人类遗传变异对年龄敏感性表型的影响。