Gene expression in sweet orange infested by Lecanicillium psalliotae

To verify the pathogenicity of Lecanicillium psalliotae invasive pathogens on tsearch leaves, Lecanicillium psalliotae was identified by isolation and purification. sweet orange leaves were infested with it. The results of the experiments showed that 15 days after Lecanicillium psalliotae infested the leaves of sweet orange, yellow spots grew around the pores and irregular yellow spots appeared on both sides of the leaf veins. This was highly similar to the disease in the field, suggesting that Lecanicillium psalliotae is the causal agent of the yellow spots on sweet orange leaves that cause the leaves to wilt and fall off. In previous studies, Verticillium cutaneum was mainly identified as a biological control agent and a suspected pathogen. In this study, the pathogenicity of Verticillium cutaneum was verified for the first time as a causal agent of leaf spot disease of plants. Overall design: Sweet orange leaves of uniform size and color, free from mechanical damage, were disinfected on an ultra-clean bench with 4% sodium hypochlorite solution and placed in sterilized petri dishes lined with sterile paper. Then, six small holes were poked on the front of the leaves with a sterile toothpick. In each hole, 3 µl of Lecanicillium psalliotae spore suspension was injected as the treatment group and an equal amount of sterile water was injected as the control group. 3 leaves were set up in both the treatment and control groups. Petri dishes were sealed with sealing film and incubated in the dark at 26°C for 14 days. After the appearance of obvious disease, 2cm of leaves around the sweet orange leaves were collected and stored in liquid nitrogen.