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Strand-specific RNA-seq analysis after Ssrp1 knockout in embryonic stem cells

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NIAID Data Ecosystem2026-03-12 收录
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Endogenous retrovirus MERVL is specifically expressed in a minority of embryonic stem cells. To determine the restrain mechanism of MERVL, we knocked out Ssrp1 and analyzed the effect on the expression of transposable elements and coding genes. We knocked out Ssrp1 in E14 by CRISPR/Cas9 System. We performed strand-specific RNA-seq analysis in order to exam the transcriptome after Ssrp1 KO.

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2020-10-26
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