RNA sequencing of e15.5 pancreas from Wild Type, Blinc1-/- and Blinc+/- mice

We report the transcriptome changes that result of the genomic deletion of one or two alleles of an islet-specific long non-coding RNA (Blinc1) in isolated pancreas from e15.5 mouse embryos. Pancreas from e15.5 embryos were dissected and total RNA extracted. Libraries were prepared from total RNA (RIN>8) with the TruSeq RNA prep kit (Illumina) and sequenced using the HiSeq2000 (Illumina) instrument. More than 20 million reads were mapped to the mouse genome (UCSC/mm9) using Tophat (version 2.0.4) with 4 mismatches and 10 maximum multiple hits. Significantly differentially expressed genes were calculated using DEseq.本研究报道了e15.5天小鼠胚胎分离胰腺中，胰岛特异性长链非编码RNA（long non-coding RNA，Blinc1）的一个或两个等位基因发生基因组缺失所诱导的转录组变化。实验首先解剖分离e15.5天小鼠胚胎的胰腺并提取总RNA；以RNA完整性数（RNA Integrity Number，RIN）>8的总RNA为起始材料，使用TruSeq RNA建库试剂盒（Illumina）构建测序文库，随后通过HiSeq2000（Illumina）测序平台完成高通量测序。利用Tophat（版本2.0.4）将超过2000万条测序reads比对至小鼠基因组（UCSC/mm9版本），比对参数设置为允许4个错配碱基及最多10个多重比对位点；最终采用DEseq软件计算得到显著差异表达基因。