Additional file 1: of Lack of detection of a human placenta microbiome in samples from preterm and term deliveries

Table S1. Human subjects studied. AA indicates African American, SVD is spontaneous vaginal delivery, (P)PROM is (preterm) premature rupture of the membranes. Table S2. Description of sample types. Table S3. Summary of samples analyzed by 16S rRNA marker gene sequencing and shotgun metagenomic sequencing. Table S4. Statistical analysis of 16S rRNA gene qPCR data. CT values were compared using the Kruskal-Wallis Test with Dunn’s Post-test. The comparison between the placenta samples and Air Swabs show a significant difference (p < 0.05), however, the Air Swab samples had lower CT values than placenta samples, and therefore higher bacterial counts. Table S5. Statistical analysis of 16S rRNA gene qPCR data of the term birth groups. CT values were compared using the Kruskal-Wallis Test with Dunn’s Post-test. Table S6. Statistical analysis of 16S rRNA gene qPCR data of the preterm birth groups. CT values were compared using the Kruskal-Wallis Test with Dunn’s Post-test. Table S7. Statistical analysis of 16S rRNA gene qPCR data of the birth groups diagnosed with chorioamnionitis. CT values were compared using the Kruskal-Wallis Test with Dunn’s Post-test. Table S8. Statistical analysis of 16S rRNA gene qPCR data of the birth groups not diagnosed with chorioamnionitis. CT values were compared using the Kruskal-Wallis Test with Dunn’s Post-test. Table S9. Statistical analysis of 16S rRNA gene qPCR data of the samples purified with the PowerSoil extraction kit. CT values were compared using the Kruskal-Wallis Test with Dunn’s Post-test. Table S10. Statistical analysis of 16S rRNA gene qPCR data of the samples purified with the UltraClean extraction kit. CT values were compared using the Kruskal-Wallis Test with Dunn’s Post-test. Table S11. Read counts for sequences acquired for each sample in the 16S rRNA marker gene sequencing and shotgun sequencing studies. Table S12. P-values for comparisons among groups as analyzed using Adonis permutation tests applied to the 16S rRNA marker gene sequencing data. All values were corrected for multiple comparisons. The comparisons tested are indicated above the columns; the samples tested are in the rows. Table S13. Sequences from metagenomic analysis of placenta samples, showing the most abundant sequences aligning to Methanosarcina mazei and Alteromonas mediterranea. Table S14. Kraken classified taxa that are found in placenta samples after removing those that are found in the negative controls. The counts are the sums of classified reads within the group. Only taxa with at least two classified reads are shown. Table S15. Kraken classified taxa that are unique to placentas from term births and absent from placentas from preterm births (reads found in negative controls were not removed). The counts are the sums of classified reads within the group. Only taxa with at least two classified reads are shown. Table S16. Kraken classified taxa that are unique to placentas from preterm births and absent from placentas from term births (reads found in negative controls were not removed). Only taxa with at least two classified reads are shown. Table S17. Oligonucleotides used in this study. (XLS 158 kb)