Gene expression profile at single cell level of aortic endothelial cells (ECs) from endothelial-specific Epas1 knockout mice (Epas1fl/fl, Cdh5Cre/+) and controls (Epas1fl/fl, Cdh5+/+)

Single cell RNA sequencing (scRNA-seq) was used to analyse the diversity of ECs in the mouse aorta, as well as the effect of endothelial-specific Epas1 deletion on aortic endothelial cells. The aorta from Epas1 EC-KO mice and Epas1 EC-WT mice was dissected and digested. Single endothelial cells were isolated by Fluorescence-activated cell sorting (FACS), selecting CD45-, CD31+ and Topro3- cells. Cells were analyzed using scRNAseq.