Gene expression profile at single cell level of thymic stromal cells

Thymic atrophy and the progressive immune decline that accompanies it is a major health problem, chronically with age and acutely with immune injury. We used single cell RNA sequencing (scRNA-seq) to analyze the diversity of mesenchymal niche cells (ThyMC) in human and murine thymi. Human (n=3) and murine (n=15) thymi were disaggregated, CD45- enriched and profiled using the Chromium Single Cell 3’ Library and Gel Bead Kit v2 and v3 (10X Genomics).