TRAP-seq of NTS/AP Calcr neurons

To identify transcripts enriched in hindbrain Calcr neurons, we employed translated ribosome affinity purification combined with RNA-seq (TRAP-seq). Overall design: Calcr-Cre mice were either crossed to a R26 Cre-dependent EGFP:L10a mouse line or injected with a Cre-dependent AAV expressing EGFP:L10a in the nucleus of the solitary tract and area postrema (NTS/AP). Ribosome-associated (Bead) and supernatant (Sup) polyA RNA were then isolated for library construction and sequencing.