Single-Cell Transcriptomic Characterization of Microscopic Colitis

Patients with microscopic colitis (MC), chronic diarrhea without histological signs of MC, or unaffected controls were profiled using single-cell RNA sequencing (scRNA-seq; inDrops and 10X Genomics were used). One profiled patient without clinical symptoms was found to have histological signs of MC (asymptomatic MC). Some of the subjects from dbGaP study phs003418 are controls in this study. T-Cell Receptor sequencing (TCR-Seq) was also performed on a subset of patients. Individual transcriptomes were analyzed to characterize the cellular profile associated with MC and search for mediators of disease pathology. Raw reads and processed count matrices will be available through dbGaP.]]>
Inclusion Criteria:Ability to give informed consentAbility and willingness to comply with all patient visits and study-related proceduresAbility to understand and complete all study-related materials and questionnairesAt least 18 years of agePatients with suspected microscopic colitis (or inclusion criteria 6, 7 or 8)Patients that have been previously treated for microscopic colitis that are being seen for possible relapse (or inclusion criteria 5 or 7)Patients with chronic diarrhea who have been ruled out for microscopic colitis (or inclusion criteria 5 or 6)Healthy patients with no GI diseases or symptoms scheduled to receive a screening colonoscopy Exclusion Criteria:Inability to provide informed consentInability or unwillingness to comply with all patient visits and study-related proceduresInability to understand and complete all study-related materials and questionnairesPatients with a known diagnosis of colorectal cancerPatients with a known bleeding disorder, acute disease, or those that are awaiting transplantationPatients who have taken antibiotics in the last two weeks.Female subjects who are pregnant or nursing.Patients with a history of IBDPatients on anticoagulants]]>