Effect of 6-azasteroids on Mycobacterium tuberculosis wild type and 6-azasteroid resistant mutants. Effect of 6-azasteroids on Mycobacterium tuberculosis wild type and 6-azasteroid resistant mutants

Transcriptional profiling of Mycobacterium tuberculosis CDC1551 and 6-azasteroid resistant mutants ASR1 and ASR2 with treatment of 6-azasteroids, 4a and 6a, for 6 hours. Overall design: Three different strains, wild type CDC1551 and two clones resistant to compound 6a, ASR1 and ASR2, were grown to OD~0.6 and treated with 30 μM of 4a or 6a or vehicle control for 6h. Total RNA was extracted and processed for RNAseq.