Effect of knockout of Samsn1 on gene expression during E.coli infection in Raw264.7 cells

In sepsis, infection and immunosuppression occur simultaneously. We currently found that Samsn1 expression is increased on myeloid cells in sepsis. And, Samsn1 is currently recognized as a negative regulator of immune response. So we constructed Samsn1 knockout Raw264.7 cells to study the effect of Samsn1 on macrophage function and the gene expression of macrophages with infection. Overall design: To investigate the functional effects of Samsn1 on macrophages, we constructed the Samsn1 knockout Raw264.7 cell line by Crispr-cas9 technology After we had treated the cells with E. coli, RNA-seq of 4 different group of cells were used for gene expression profiling analysis Comparative gene expression profiling analysis of RNA-seq between different groups