James R. LaFountain, Rudolf Oldenbourg (2011) CIL:10725, Nephrotoma, Nephrotoma suturalis, primary spermatocyte. CIL. Dataset

Movie of anaphase A and B of meiosis I in primary spermatocytes of the crane-fly Nephrotoma suturalis.
Time-lapse polarization microscopy using a Nikon
Microphot SA, equipped for liquid crystal polarized light microscopy
(LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 2.0x zoom lens. Images captured every 30 sec over 60 min using a QImaging Retigo EXi CCD camera. Raw images were processed
using 4-frame algorithm (Shribak and Oldenbourg, 2003). The time series of images on which the movie is based is included in this grouped set.

本数据集收录了蜻蜓目昆虫河蝇（Nephrotoma suturalis）初级精母细胞减数分裂I期中A和B期的动态电影。采用尼康Microphot SA显微镜，配备液晶偏振光显微镜系统（LC-PolScope，CRi，Woburn，马萨诸塞州），搭载60x/1.4 PlanApo油浸物镜，1.4 NA油浸聚光镜，以及2.0倍放大镜头。图像以每30秒捕获一次的频率，持续60分钟，由QImaging Retigo EXi CCD相机捕捉。原始图像经过4帧算法（Shribak和Oldenbourg，2003年）处理。基于此图像时间序列的电影已包含在本分组集中。