Nitrospira tepida DNF complete genome. Complete genome of Nitrospira tepida DNF isolated from activated sludge

The genus Nitrospira represents the dominant nitrite-oxidizing clade in most wastewater treatment plants globally and several Nitrospira strains have been isolated from activated sludge. With a pre-enrichment in alternating nitrifying and denitrifying conditions as well as elevated cultivation temperatures, we could isolate and re-enrich a novel species namely Nitrospira tepida DNF. This moderately thermophilic Nitrospira strain is only distantly related to other Nitrospira and forms a novel lineage (lineage VII) together with few highly similar environmental 16S rRNA gene sequences predominantly detected in warm wastewater systems or oxygen limited reactors.