Impact of a pro-inflammatory stimulus on the miRNA composition of extracellular vesicles (EV) from stem cells from apical papilla (SCAP)

To investigate the impact of a pro-inflammatory stimulus (TNFa and IFN?, 20 ng/ml) on miRNA in SCAP-EV, we isolated EV from non-activated and activated SCAP and then extracted miRNA Overall design: Comparative miRNA expression between EV from non-activated SCAP and EV from activated SCAP (activation with TNFa and IFN?). We replicated this experiment 3 times.