Comparison of Kcat values model and values from literature

Comparison of Kcat values model and values from literature. Model values are based on Vmax enzyme parameters (maximum activity per enzyme molecule).
Literature values are largely based on whole cell enzyme extract assays and do not take into account allosteric control. In addition activity is measured at varying time points and varying conditions. The error based on differences in enzyme concentrations at different time points and the error in protein copy number measurements is taken into account in the comparison. In addition Sensitivity analysis show the importance of different Vmax values on flux through glycolysis.

本数据集对酶动力学模型中的Kcat值与文献中的值进行了比较。模型值基于Vmax酶参数（每酶分子的最大活性）。而文献中的值主要基于全细胞酶提取物试验，未考虑变构调控的影响。此外，活性测量在多个时间点和不同条件下进行。比较中考虑了不同时间点酶浓度差异以及蛋白质拷贝数测量误差。此外，敏感性分析揭示了不同Vmax值对糖酵解通量重要性。