Expression data in U2OS cells transfected with control-, DBP5-, GLE1- or IPPK-siRNA

GLE1, DBP5 and inositol hexakisphosphate (IP6) function in mRNA export at the cytoplasmic face of the NPC in eukaryotic cells. It is believed that these three factors have a major and consistent role for the mRNA export. We performed RNA microarray analysis to detect the effect and function of DBP5, GLE1 and IPPK (IP6 synthetic enzyme) on the mRNA expression. The expression of some mRNA subsets (e.g. cell cycle, DNA replication) was similarly suppressed. However, GLE1 knockdown specifically attenuates the mRNA expression of factors participated in Sonic hedgehog signaling. On the other hand, several factors function to immunoresponse was downregulated in DBP5 or IPPK knocked-down cells. These results imply that DBP5, GLE1 and IP6 have a conserved and individual function in the mRNA expression. Cytoplasmic RNA was prepared in U2OS cells transfected with control-, DBP5-, GLE1- or IPPK-siRNA and analyzed by gene expression array.