Zoo elephant metagenomic Kraken2 reports
收藏DataONE2022-12-08 更新2025-08-02 收录
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Non-invasive biological samples benefit studies that investigate rare, elusive, endangered, and/or dangerous species. Integrating genomic techniques that use non-invasive biological sampling with advances in computational approaches can benefit and inform wildlife conservation and management. We use non-invasive fecal DNA samples to generate low- to medium-coverage genomes (e.g., >90% of the complete nuclear genome at 6 X-fold coverage) and metagenomic sequences, combining widely available and accessible DNA collection cards with commonly used DNA extraction and library-building approaches. DNA preservation cards are easy to transport and can be stored non-refrigerated, avoiding cumbersome and/or costly sample methods. The genomic library construction and shotgun sequencing approach did not require enrichment or targeted DNA amplification. The utility and potential of the generated data generated were demonstrated through genome-scale analysis and metagenomics of zoo and free-ranging..., These files contain the Kraken2 output for each of the zoo elephants included in the manuscript, \"Blending methods for non-invasive fecal DNA opens whole genome and metagenomic analyses in wildlife biology\".
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创建时间:
2025-07-20



