RNA-sequencing of inguinal white adipose tissue (iWAT) from kynurenine 3-monooxygenase (Kmo) knockout mice and wild-type littermates on chow and high-fat diets.

To investigate if chronically elevated circulating kynurenic acid (KYNA) and reduced quinolinic acid (QUIN) levels affect whole-body energy metabolism, we used a knockout-first gene targeting strategy to eliminate Kmo gene expression in mice. We conducted a series of metabolic assessments on male and female mice fed either a chow or high-fat diet (HFD) and performed RNA-sequencing on the inguinal white adipose tissue (iWAT) from male Kmo-defficient mice and their wild-type littermate controls on chow and high-fat diets. RNA sequencing analysis of inguinal white adipose tissue from whole-body male Kmo knockout mice and wild-type littermate controls on chow and high-fat diets (n = 4, age = 6-7 months). Total RNA was extracted using TRI reagent (Sigma-Aldrich, T9424) according to the manufacturer's instructions. RNA was further purified, and DNase treated (Zymo, R1013), and integrity confirmed prior to sequencing (Agilent Technologies, 2100 Bioanalyzer), with an RNA integrity number (RIN) greater than 7 as a threshold. Samples were purified by poly-A enrichment before library preparation using the Illumina NovaSeq X Plus Series platform with a paired-end 150 base pair strategy.为探究慢性升高的循环犬尿喹啉酸（KYNA）与喹啉酸（QUIN）水平降低是否会对全身能量代谢产生影响，我们采用敲除优先型基因靶向策略，敲除小鼠体内的Kmo基因表达。我们对饲喂普通饲料或高脂饮食（HFD）的雌雄小鼠开展了一系列代谢评估，并对饲喂普通饲料与高脂饮食的全身Kmo敲除雄性小鼠及其野生型同窝对照的腹股沟白色脂肪组织（iWAT）进行RNA测序（RNA-seq）。本数据集对应上述样本的RNA测序分析结果，每组样本量n=4，小鼠年龄为6~7月龄。总RNA采用TRI试剂（Sigma-Aldrich，货号T9424），严格按照厂商说明书进行提取；随后对RNA进行进一步纯化并使用DNase处理（Zymo，货号R1013），测序前通过安捷伦2100生物分析仪（Agilent Technologies, 2100 Bioanalyzer）验证RNA完整性，以RNA完整性数（RIN）≥7作为合格阈值。文库构建前通过polyA富集对样本进行纯化，随后采用Illumina NovaSeq X Plus系列测序平台，以双端150bp的测序策略完成测序。