ChIP-Seq analysis of H3K4me3 and H3K27ac histone marks without and with METTL8 knockdown

This project investigates the role METTL8 in human glioblastoma stem cells. In order to understand the transcriptomic changes upon deletion of METTL8, we performed this ChIP-seq and further downstream analyses. From this, we hope to identify novel methods to target glioblastoma stem cells via manipulation of METTL8. ChIP-seq analysis of H3K4me3 and H3K27ac histone marks from glioblastoma stem cell specimens (TS-576) following treatment with either non-targeting RNA (shNT) , targeting RNA sh515 or targeting RNA sh516.