five

Normal blood cell subtypes

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NIAID Data Ecosystem2026-03-07 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE4889
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We used antibodies coupled to magnetic beads (MACS) to purify cell types out of whole blood from healthy donors (CD4+ T cells, CD8+ T cells, and B cells). We also profiled whole blood (collected in Pax tubes) and Peripheral Blood Mononuclear cells obtained by Ficoll-Paque based separation. RNA was extracted using Trizol, amplified linearly twice, labeled with Cy5 and co-hybridized with amplified Stratagene human reference (labeled with Cy3). A reference experiement design type is where all samples are compared to a common reference. Keywords: reference_design Using regression correlation
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2012-03-16
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